Device

Part:BBa_K4720104:Design

Designed by: Jelle de Boer   Group: iGEM23_Groningen   (2023-10-12)


J23100+RBS+FleQ+TT+Pcl+Ppel+RBS+Lux


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7183
    Illegal BamHI site found at 233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3220
    Illegal SapI site found at 5359


Design Notes

A XbaI restriction site needed to be removed from the lux reporter in order for it to be a iGEM RFC[10] compatible part.


Source

This is a combination of our own parts: BBa_K4720017 and BBa_K4720105.

References